| System suitability | tR (minutes) | 5.123 | 8.758 | 11.308 |
| %RSDtR | 0.14 | 0.10 | 0.09 |
| Peak area Spic (μAU.s) | 921378 | 230373 | 4247032 |
| %RSDSpic | 0.15 | 0.14 | 0.15 |
| Apparent theoretical plate number | 14661 | 52046 | 53666 |
| Tailing factor | 1.1 | 1.0 | 1.1 |
| Rs | 2.8 | 22.8 | 14.9 |
| Specificity | | Figure 4 | Figure 4 | Figure 4 |
| Linearity | Regression correlation equation | ŷ=38201.8689x | ŷ=24344.3812x | ŷ=54915.8383x |
| Correlation coefficients R | 0.9997 | 0.9996 | 0.9996 |
| Linear concentration range (ppm) | 12.52 – 37.55 | 5.00 – 15.01 | 40.01 – 120.02 |
| Accuracy | Average recovery rate (%) | 99.96 | 99.57 | 99.31 |
| %RSD (n = 9) | 0.27 | 0.12 | 0.26 |
| Repeatability | Average content of test sample (%) | 99.58 | 101.05 | 101.15 |
| %RSD (n = 6) | 0.51 | 0.68 | 0.54 |
| Intermediate precision | Average content of test sample (%) | 99.61 | 101.21 | 101.43 |
| %RSD (n = 6) | 0.53 | 0.57 | 0.52 |
| F test và t test (n1 = 6 và n2 = 6; α = 0.05) | There is no significant difference | There is no significant difference | There is no significant difference |
| Range | (ppm) | 20.02 – 30.04 | 8.01 – 12.01 | 64.01 – 96.01 |
| Robustness | System suitability of standard mixture solution; RSD (%) | Buffer amonium dihydrophos phate pH 3.2 | RSD % peak area | 0.25 | 0.15 | 0.14 |
| RSD % retention time | 0.71 | 0.79 | 0.68 |
| Buffer amonium dihydrophos phate pH 2.8 | RSD % peak area | 0.27 | 0.50 | 0.21 |
| RSD % retention | 0.55 | 0.85 | 0.69 |